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Cedervind, Holly (2004) Glycogen content in different muscle fibre types before and after lengthy anaesthesia in horses. Other thesis, SLU.

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General anaesthesia in horses is often followed by postoperative complications, including myopathy. Many colic patients are compromised by preoperative stressors, such as starvation, trailer rides, physical stress and pain, plus postoperative physical stress in connection with standing up after recumbency. These factors may influence muscle glycogen stores, since glycogen is an important substrate source in metabolism. The aim of this study was to investigate muscle glycogen content with histochemical techniques, before and after lengthy anaesthesia in both healthy horses and in colic patients. Seven colic patients and 5 healthy warmblooded trotters were anaesthetized (duration > 3 hr). Patients did not eat (9-51 hr) prior to surgery and had been transported large distances to surgery (53 -320 km). Healthy horses were denied food (12 h) prior to anaesthesia. Choice of premedication and induction agents was left to the anaesthetist. Anaesthesia was maintained with isoflurane with horses in dorsal recumbency. M. gluteus medius was biopsied in all horses at induction, end of anaesthesia and in some horses, 24 hours after anaesthesia. Samples were cut in a cryostat and stained for myosin ATP-ase activity after pre-incubation at pH 4.6, to identify type I, IIA and IIB fibres, and with Periodic Acid-Schiff's reagent, in order to subjectively evaluate 'low' or 'high' glycogen content in fibres. Healthy horses and two patients had a high glycogen content in all fibre types at all biopsy times. Three patients had a high glycogen content in most fibres at induction of anaesthesia, but had a few low staining fibres at end of anaesthesia. A low glycogen content in most fibres (primarily type I fibres) was seen at induction of anaesthesia in the other four patients. In three of these horses, type IIB fibres also had a low glycogen content. Changes in glycogen content were still evident at end of anaesthesia and even 24 hours after standing up in recovery. The horses with a poor preoperative physical status had many fibres with low glycogen content. This study has shown that glycogen availability in muscle fibres may be an important factor to consider in anaesthesia and in the postoperative recovery period, and that this could be related to preoperative stress factors.

Item Type: Thesis (Other)
Keywords: equine, anaesthesia, colic, muscle glycogen, histochemistry
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Dept. of Large Animal Clinical Sciences
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Holly Cedervind
Date Deposited: 14 Jan 2004
Last Modified: 18 Aug 2015 09:28
URI: http://ex-epsilon.slu.se/id/eprint/55

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