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Junttila, Laura (2009) Effekt av spädningsvätska på spermiemembranintegritet analyserad med NucleoCounter SP-100. Other thesis, SLU.

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Abstract

This study is a part of a larger project where the aim has been to evaluate different methods to assure the quality in stallion semen, due to the fact that some stallions in horse breeding have poor semen quality. The increased use of transport semen requires new methods to ensure good semen quality. In this part of the project the viability and concentration in fresh and cooled semen was examined with NucleoCounter SP-100. A comparison between two different commercially available semen extenders, Kenney's and INRA 96, was made. The selection method Single Layer Centrifugation (SLC) was used in the study and the spermatozoal viability was compared between the selected and unselected semen. Since the NucleoCounter SP-100 is a relatively new machine a comparison was also made with flowcytometry regarding viability. Concentration measured with NucleoCounter SP-100 was compared by the use of manual counting with the Bürker chamber. The results show that no statistical difference was detected between the two different extenders regarding spermatozoal viability. On the other hand SLC had a statistically positive effect on the viability of the spermatozoa over time (p < 0,0001). A significant interaction was noticed between semen extender (Kenney's och INRA 96) and handling of semen (unselected and selected). Viability was best conserved in Kenney's selected and INRA 96 selected had the next best ability to conserve viability over time. Kenney's unselected had the lowest result of viability over time. The comparison between NucleoCounter SP-100 and flowcytometry regarding spermatozoal viability didn't show any correlation. In this part of the study only 19 ejaculates were included and a greater number of stallions and ejaculates should be used for more accurate results. Concerning the concentration measurement, the NucleoCounter SP-100 correlated very well with Bürker chamber (r = 0,93) and the significance was high (p < 0,0001). The results show that SLC has a good effect on sperm viability over time while choice of semen extender does not have any significant effect. NucleoCounter SP-100 could be used at the studs for concentration measurement but regarding viability, a larger study should be made to evaluate the NucleoCounter machine.

Item Type: Thesis (Other)
Keywords: spermiemembranintegritet, INRA 96, Kenneys, Single Layer Centrifugation, viabilitet
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Dept. of Clinical Sciences
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Laura Junttila
Date Deposited: 04 Mar 2009
Last Modified: 18 Aug 2015 10:12
URI: http://ex-epsilon.slu.se/id/eprint/3156

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