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Karlsson, Marie (2009) Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP. Other thesis, SLU.

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Abstract

Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine. With quantitative real-time PCR and antigen ELISA has the levels of the cytokines IFN-γ and IL-10 been studied in blood from SPF cats immunised with an ISCOM vaccine against FIPV and then challenged. The levels of IFN-γ and IL-10 were too low in the serum samples to be able to analyze with the ELISA used. With real-time PCR, which is a very sensitive method, all the cats in the trial expressed IFN-γ and IL-10 at one or more samplings. No apparent difference was seen between the vaccinated and control cats. Commercial ELISA can have a too high lowest detection level than what's useful when analyzing cytokines in samples from cats that have not developed FIP. For further analyzes of cytokines in very low concentrations a more sensitive method is needed, where proximity ligation assay is one possible alternative.

Item Type: Thesis (Other)
Keywords: FIP, cytokiner, vaccination, PCR, ELISA
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Department of Biomedical Science and Veterinary Public Health
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Marie Karlsson
Date Deposited: 23 Jan 2009
Last Modified: 18 Aug 2015 10:11
URI: http://ex-epsilon.slu.se/id/eprint/3040

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