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Strutz, Hanna (2007) Bedömning av spermiemotilitet i färsk, kyld samt selekterad hingstsperma med Qualisperm. Other thesis, SLU.

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Abstract

This EEF-project is a part of a larger project at SLU, Division of Reproduction, Dept of Clinical Science, with the intention of developing and evaluating new methods for the assessment of stallion semen quality. Over the last 10 years there has been an increase in the use of artificial insemination with cooled semen in Swedish horse breeding. At the same time, foaling rates have decreased. This has lead to a need for new and better methods of assessing the quality of stallion semen. This EEF-project was based on an objective method to calculate motility, with QualispermTM, in fresh and cooled stallion semen, and after selection. Today sperm motility evaluation is mainly done subjectively by visual assessment with a light microscope, and the correlation between these motility results and fertility have varied. Therefore, there have been a number of objective methods designed, for example CASA (Computer Assisted Semen Analysis) where QualispermTM (Biophos AG, Switzerland) is a new system based on different principles compared to earlier CASA-systems. While other CASA-machines takes a series of photographs and the trajectories are used to separate the sperm tracks into categories of different motility patterns, QualispermTM determines the number of particles (spermatozoa) crossing fields of view, yielding a regression fluctuation algorithm of sperm numbers and translation classes. Furthermore it analyzes thousands of spermatozoa per sample compared to other CASAmachines where often only a couple of hundred spermatozoa are measured. QualispermTM is also easier to use than other CASA-machines, which makes it more suitable for use out in the field. This EEF-project was carried out on the national stud at Flyinge during three weeks in the summer of 2007. Three or four ejaculates /stallion from 10 stallions where analysed during 3 days for subjective and objective motility in fresh and cooled semen and after selection by centrifugation. The semen was kept both in room temperature and chilled at + 5 ° C, to study how the temperature would affect the motility. The statistical analysis showed that there was a significant correlation between the subjective and objective motility. There was also a significant difference in motility depending on how the semen was handled (temperature and selection through colloid centrifugation). The results indicated that the motility was best preserved by selection and storage at + 5 ° C. There were also individual differences between stallions, concerning the time their spermatozoa kept their motility. Analysing spermatozoa with an atypical motility before and after centrifugation showed that this method effectively selected progressively moving spermatozoa. This study showed that QualispermTM could be a good complement to traditional methods in the field in the future, but some further development of the machine must first be done. There is still some subjectivness built in the method, since the user chooses the spot for the analyze, and there is also a need for training before using QualispermTM

Item Type: Thesis (Other)
Keywords: Qualisperm, hingstsperma, motilitet
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Dept. of Clinical Sciences
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Hanna Strutz
Date Deposited: 01 Feb 2008
Last Modified: 18 Aug 2015 09:58
URI: http://ex-epsilon.slu.se/id/eprint/2195

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