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Nygren, Therese (2008) Trombocyträkning hos katt. Other thesis, SLU.

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Abstract

Platelet aggregation is a major and common problem in blood samples from cats. This greatly affects the accuracy of counting of the platelets. Because of the difficulties in counting feline platelets, results for patients and reliable reference values for cat platelets are currently so inaccurate as to be almost useless in diagnosis of thrombocytopenia. In this study we used an effective method to prevent platelet aggregation. Blood was collected in CTAD test tubes and prostaglandin E1 was added to the blood sample. Blood samples were collected from nine healthy cats 1-10 years of age. The blood was analysed within 1-4 hours at the laboratory of clinical chemistry. The platelets were counted both manually and in automated cell counters. Three cell counters were used. A Sysmex XT 2000Vi counted platelets both optically with a laser system and by using impedance analysis. The Siemens Advia 2120 used an optical laser system to count platelets. The Cell-Dyn 3500 counted platelets by impedance. The blood samples were checked for platelet aggregation in the hemocytometer (glass chamber) and on the blood smears. Platelet aggregation was prevented when adding prostaglandin E1 to blood samples directly after they were collected in CTAD test tubes. Since no or only a few small platelet aggregates were present in the blood samples after addition of prostaglandin E1 to the CTAD test tubes, the platelet counts aquired from the nine cats can be used as reference values for the instruments and manual method used in this study. The reference values in this study were 180-493x109/L for Sysmex XT 2000Vi optical analysis, 182-525 x109/L for Siemens Advia 2120 and 161-380 x109/L for the manual platelet counts. Moreover the CTAD-prostaglandin E1 method may be used with feline patients to obtain accurate platelet counts and allow better diagnosis.

Item Type: Thesis (Other)
Keywords: trombocyter, katt, aggregering, prostaglandin E1, referensvärden, trombocytantal
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Dept. of Clinical Sciences
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Therese Nygren
Date Deposited: 18 Jan 2008
Last Modified: 18 Aug 2015 09:58
URI: http://ex-epsilon.slu.se/id/eprint/2141

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