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Elving, Josefine (2006) Immunostimulatory DNA. Other thesis, SLU.

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Nucleotide sequences containing CpG-motifs are recognized as immunomodulators in pigs among other species. Phosphodiester oligodeoxynucleotides (ODNs), such as ODN H, have been demonstrated to be potent inducers of interferon-alpha (INF-alpha) in porcine blood mononuclear cells (poPBMC) provided they are pre-treated with Lipofectin®. In the present study the IFN-alpha inducing activity of ODN H was demonstrated to be altered after addition of poly-G sequences at the 5' and/or 3' end and by increasing the number of nucleotides (nt) in the base-pairing sequence of the ODN. Alterations that facilitated the formation of secondary structures reduced the need for pre-treatment with Lipofectin®. Furthermore, deliberate destruction of secondary structures by heat-treatment of ODN 2216 reduced the IFN-alpha inducing capacity of that ODN. Thus, results suggest that the stimulatory activity of the ODNs is dependent on secondary structure formation. Other ODNs are recognized as potent inhibitors of the INF-alpha production in poPBMC. The ODN PCV2/1 corresponding to a 20 nt long sequence from the genome of PCV2, has been identified as such an inhibitory ODN. Further, it was demonstrated that the inhibitory capacity of ODN PCV2/1 not depend on the presence of a central CpG-motif. Further, results from the in vitro studies show a significant difference between ODN PCV2/1 found in the genome of PCV2 from healthy pigs in Sweden and ODN PCV2/1S1, which predominates in pigs from farms with PMWS in Sweden. However, the biological significance of these findings remain to be elucidated.

Item Type: Thesis (Other)
Keywords: interferon, porcine circovirus, PCV-2, CpG, secondary structure, inhibitory, PCR, ODN
Subject (faculty): Faculty of Veterinary Medicine and Animal Science > Dept. of Molecular Biosciences
Divisions: SLU > Faculty of Veterinary Medicine and Animal Science
Depositing User: Josefine Elving
Date Deposited: 25 Jul 2006
Last Modified: 18 Aug 2015 09:41
URI: http://ex-epsilon.slu.se/id/eprint/1089

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